In vitro respiration rates of the Mytilidae Aulacomya atra and Mytilus chilensis from the fjord Comau, Chile, 2012

Respiration rates of Aulacomya atra and Mytilus chilensis were measured in vitro. Specimens covering the entire size range were sampled from different sides in the fjord Comau and cleaned from the fouling community. Mussels were sampled in the intertidal zone (Mytilus chilensis) and the shallow subtidal zone (Aulacomya atra). The sampling depth ranged from 0 to 20 m below Mean Sea Level (MSL). Prior to the respiration experiments all test bivalves were maintained under laboratory conditions for five days, to assure similar feeding conditions and acclimatization of all animals. Mussel husbandry was performed in tanks (100 l) constantly refilled (10 l/min) with filtered seawater (decreasing mesh sizes: 100, 50, 25, 10 µm) pumped from the fjord (20 m water depth). Single mussels were incubated in respiration chambers for 1 h at 12-13 °C. Oxygen concentration was measured after the experimental period using an oxygen optode (Hach HQ40d dual input multi parameter Meter + IntelliCAL LDO101 Standard Luminescent Dissolved Oxygen sensor). The volume of the chamber was adjusted to the size of the mussel to ensure a reduction of the oxygen concentrations to 80-90% of the initial value. This dataset was generated in the frame of the co-operation between the Huinay Scientific Field Station (http://www.huinay.cl) and the Alfred Wegener Institute, Helmholtz Center for Polar and Marine Research (http://www.awi.de).

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